gram’s method

noun (sometimes lowercase)

  1. a method of staining and distinguishing bacteria, in which a fixed bacterial smear is stained with crystal violet, treated with Gram’s solution, decolorized with alcohol, counterstained with safranine, and washed with water.


  1. bacteriol a staining technique used to classify bacteria, based on their ability to retain or lose a violet colour, produced by crystal violet and iodine, after treatment with a decolorizing agentSee also Gram-negative, Gram-positive

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